Cargando…
Immunoprecipitation of Reporter Nascent Chains from Active Ribosomes to Study Translation Efficiency
The study of translation is important to the understanding of gene expression. While genome-wide measurements of translation efficiency (TE) rely upon ribosome profiling, classical approaches to address translation of individual genes of interest rely on biochemical methods, such as polysome fractio...
Autores principales: | Cacioppo, Roberta, Lindon, Catherine |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bio-Protocol
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518778/ https://www.ncbi.nlm.nih.gov/pubmed/37753466 http://dx.doi.org/10.21769/BioProtoc.4821 |
Ejemplares similares
-
Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification
por: Imai, Hirotatsu, et al.
Publicado: (2023) -
Nuclear translation visualized by ribosome-bound nascent chain puromycylation
por: David, Alexandre, et al.
Publicado: (2012) -
Nascent SecM Chain Outside the Ribosome Reinforces Translation Arrest
por: Yang, Zhuohao, et al.
Publicado: (2015) -
Nascent chain dynamics and ribosome interactions within folded ribosome–nascent chain complexes observed by NMR spectroscopy
por: Burridge, Charles, et al.
Publicado: (2021) -
Nonorthogonal tRNA(cys)(Amber) for protein and nascent chain labeling
por: Koubek, Jiří, et al.
Publicado: (2015)