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Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells

Prime editing introduces single-nucleotide polymorphism changes, small deletions, or insertions at a specific genome site without double-stranded DNA breaks or the need for the donor template. Here, we present a protocol to design, conduct, and evaluate prime editing in human pluripotent stem cells....

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Detalles Bibliográficos
Autores principales: Wu, Youjun, Sidharta, Mega, Zhong, Aaron, Persily, Benjamin, Li, Mu, Zhou, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519846/
https://www.ncbi.nlm.nih.gov/pubmed/37738119
http://dx.doi.org/10.1016/j.xpro.2023.102583