Quantitation of mitotic cells in the neural tube of zebrafish embryos using automated nuclei counting

Here, we provide a protocol to automate the quantification of the number of phospho-histone H3-positive cells in the developing nervous system of zebrafish using a custom MATLAB script to identify labeled nuclei. We describe steps for fixation, immunolabeling, and imaging of zebrafish embryos. We th...

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Detalles Bibliográficos
Autores principales: Emond, Michelle R., Jontes, James D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519849/
https://www.ncbi.nlm.nih.gov/pubmed/37738116
http://dx.doi.org/10.1016/j.xpro.2023.102596

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519849/
https://www.ncbi.nlm.nih.gov/pubmed/37738116
http://dx.doi.org/10.1016/j.xpro.2023.102596

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