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Quantitation of mitotic cells in the neural tube of zebrafish embryos using automated nuclei counting
Here, we provide a protocol to automate the quantification of the number of phospho-histone H3-positive cells in the developing nervous system of zebrafish using a custom MATLAB script to identify labeled nuclei. We describe steps for fixation, immunolabeling, and imaging of zebrafish embryos. We th...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519849/ https://www.ncbi.nlm.nih.gov/pubmed/37738116 http://dx.doi.org/10.1016/j.xpro.2023.102596 |