Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices

Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection. We then detail realization of slices and patch-clamp recordings. While we use, as an example, tanycytes o...

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Detalles Bibliográficos
Autores principales: Lhomme, Tori, Prévot, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519851/
https://www.ncbi.nlm.nih.gov/pubmed/37733593
http://dx.doi.org/10.1016/j.xpro.2023.102571

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519851/
https://www.ncbi.nlm.nih.gov/pubmed/37733593
http://dx.doi.org/10.1016/j.xpro.2023.102571

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