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Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices
Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection. We then detail realization of slices and patch-clamp recordings. While we use, as an example, tanycytes o...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519851/ https://www.ncbi.nlm.nih.gov/pubmed/37733593 http://dx.doi.org/10.1016/j.xpro.2023.102571 |