Protocol for real-time imaging of membrane fission by mitofissin

Yeast mitofissin Atg44 is a mitochondrial intermembrane space protein that causes membrane fission required for mitophagy. Here, we present a protocol for observing Atg44-mediated membrane fission. We describe steps for recombinant Atg44 purification, lipid nanotube preparation as model membranes, a...

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Detalles Bibliográficos
Autores principales: Maruyama, Tatsuro, Noda., Nobuo N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520655/
https://www.ncbi.nlm.nih.gov/pubmed/37738122
http://dx.doi.org/10.1016/j.xpro.2023.102590

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520655/
https://www.ncbi.nlm.nih.gov/pubmed/37738122
http://dx.doi.org/10.1016/j.xpro.2023.102590

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