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The Impact of Chemical Fixation on the Microanatomy of Mouse Organotypic Hippocampal Slices
Chemical fixation using paraformaldehyde (PFA) is a standard step for preserving cells and tissues for subsequent microscopic analyses such as immunofluorescence or electron microscopy (EM). However, chemical fixation may introduce physical alterations in the spatial arrangement of cellular proteins...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521345/ https://www.ncbi.nlm.nih.gov/pubmed/37709524 http://dx.doi.org/10.1523/ENEURO.0104-23.2023 |