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A high-throughput protocol for deamination of long single-stranded DNA and oligo pools containing complex sequences

Cytidine deaminases as DNA mutators play important roles in immunity and genome stability. Here, we present a high-throughput protocol for deamination of long single-stranded (ss) DNA or oligo pools containing complex sequences. We describe steps for the preparation of both enzyme (activation-induce...

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Detalles Bibliográficos
Autores principales: Wang, Yanyan, Zhang, Senxin, Zheng, Xiaoqi, Yeap, Leng-Siew, Meng, Fei-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522977/
https://www.ncbi.nlm.nih.gov/pubmed/37742176
http://dx.doi.org/10.1016/j.xpro.2023.102602