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A high-throughput protocol for deamination of long single-stranded DNA and oligo pools containing complex sequences
Cytidine deaminases as DNA mutators play important roles in immunity and genome stability. Here, we present a high-throughput protocol for deamination of long single-stranded (ss) DNA or oligo pools containing complex sequences. We describe steps for the preparation of both enzyme (activation-induce...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522977/ https://www.ncbi.nlm.nih.gov/pubmed/37742176 http://dx.doi.org/10.1016/j.xpro.2023.102602 |