Optimized protocol for whole-mount RNA fluorescent in situ hybridization using oxidation-mediated autofluorescence reduction on mouse embryos

Tissue autofluorescence poses significant challenges for RNA and protein analysis using fluorescence-based techniques. Here, we present a protocol that combines oxidation-mediated autofluorescence reduction with detergent-based tissue permeabilization for whole-mount RNA-fluorescence in situ hybridi...

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Detalles Bibliográficos
Autores principales: Morabito, Angela, Malkmus, Jonas, Pancho, Anna, Zuniga, Aimée, Zeller, Rolf, Sheth, Rushikesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522992/
https://www.ncbi.nlm.nih.gov/pubmed/37742180
http://dx.doi.org/10.1016/j.xpro.2023.102603

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522992/
https://www.ncbi.nlm.nih.gov/pubmed/37742180
http://dx.doi.org/10.1016/j.xpro.2023.102603

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