Cargando…
Photoswitching alters fluorescence readout of jGCaMP8 Ca(2+) indicators tethered to Orai1 channels
We used electrophysiology and Ca(2+) channel tethering to evaluate the performance of jGCaMP8 genetically encoded Ca(2+) indicators (GECIs). Orai1 Ca(2+) channel–jGCaMP8 fusions were transfected into HEK 293A cells and jGCaMP8 fluorescence responses recorded by simultaneous total internal reflection...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
National Academy of Sciences
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523504/ https://www.ncbi.nlm.nih.gov/pubmed/37729200 http://dx.doi.org/10.1073/pnas.2309328120 |