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Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro Study

In this study, we developed an osteoplastic material based on collagen–fibronectin hydrogel impregnated with siRNA molecules targeting glycogen synthase kinase 3β (GSK3β), which inhibits the osteogenic differentiation of mesenchymal stem cells. The hydrogel impregnated with polyplexes containing siR...

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Autores principales: Galitsyna, Elena V., Buianova, Anastasiia A., Kozhukhov, Vadim I., Domogatsky, Sergey P., Bukharova, Tatiana B., Goldshtein, Dmitriy V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525875/
https://www.ncbi.nlm.nih.gov/pubmed/37760805
http://dx.doi.org/10.3390/biomedicines11092363
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author Galitsyna, Elena V.
Buianova, Anastasiia A.
Kozhukhov, Vadim I.
Domogatsky, Sergey P.
Bukharova, Tatiana B.
Goldshtein, Dmitriy V.
author_facet Galitsyna, Elena V.
Buianova, Anastasiia A.
Kozhukhov, Vadim I.
Domogatsky, Sergey P.
Bukharova, Tatiana B.
Goldshtein, Dmitriy V.
author_sort Galitsyna, Elena V.
collection PubMed
description In this study, we developed an osteoplastic material based on collagen–fibronectin hydrogel impregnated with siRNA molecules targeting glycogen synthase kinase 3β (GSK3β), which inhibits the osteogenic differentiation of mesenchymal stem cells. The hydrogel impregnated with polyplexes containing siRNA GSK3β and polyethylenimine has been shown to have no cytotoxic effect: there was no statistically significant change in the cell’s viability after 7 days of incubation in its presence compared to the control group. On days 2 and 7, an increase in the level of expression of markers of osteogenic differentiation was observed, which confirms the osteoinductive qualities of the material. It has been demonstrated that the hydrogel maintains cell adhesion. Our results obtained in vitro indicate cytocompatibility and osteoinductive properties of collagen–fibronectin hydrogel impregnated with siRNA GSK3β molecules.
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spelling pubmed-105258752023-09-28 Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro Study Galitsyna, Elena V. Buianova, Anastasiia A. Kozhukhov, Vadim I. Domogatsky, Sergey P. Bukharova, Tatiana B. Goldshtein, Dmitriy V. Biomedicines Article In this study, we developed an osteoplastic material based on collagen–fibronectin hydrogel impregnated with siRNA molecules targeting glycogen synthase kinase 3β (GSK3β), which inhibits the osteogenic differentiation of mesenchymal stem cells. The hydrogel impregnated with polyplexes containing siRNA GSK3β and polyethylenimine has been shown to have no cytotoxic effect: there was no statistically significant change in the cell’s viability after 7 days of incubation in its presence compared to the control group. On days 2 and 7, an increase in the level of expression of markers of osteogenic differentiation was observed, which confirms the osteoinductive qualities of the material. It has been demonstrated that the hydrogel maintains cell adhesion. Our results obtained in vitro indicate cytocompatibility and osteoinductive properties of collagen–fibronectin hydrogel impregnated with siRNA GSK3β molecules. MDPI 2023-08-24 /pmc/articles/PMC10525875/ /pubmed/37760805 http://dx.doi.org/10.3390/biomedicines11092363 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Galitsyna, Elena V.
Buianova, Anastasiia A.
Kozhukhov, Vadim I.
Domogatsky, Sergey P.
Bukharova, Tatiana B.
Goldshtein, Dmitriy V.
Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro Study
title Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro Study
title_full Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro Study
title_fullStr Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro Study
title_full_unstemmed Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro Study
title_short Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro Study
title_sort cytocompatibility and osteoinductive properties of collagen-fibronectin hydrogel impregnated with sirna targeting glycogen synthase kinase 3β: in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525875/
https://www.ncbi.nlm.nih.gov/pubmed/37760805
http://dx.doi.org/10.3390/biomedicines11092363
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