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Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring
Electrochemical biosensors based on immobilized enzymes are among the most popular and commercially successful biosensors. The literature in this field suggests that modification of electrodes with nanomaterials is an excellent method for enzyme immobilization, which can greatly improve the stabilit...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526424/ https://www.ncbi.nlm.nih.gov/pubmed/37754120 http://dx.doi.org/10.3390/bios13090886 |
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author | Sun, Ganchao Wei, Xiaobo Zhang, Dianping Huang, Liben Liu, Huiyan Fang, Haitian |
author_facet | Sun, Ganchao Wei, Xiaobo Zhang, Dianping Huang, Liben Liu, Huiyan Fang, Haitian |
author_sort | Sun, Ganchao |
collection | PubMed |
description | Electrochemical biosensors based on immobilized enzymes are among the most popular and commercially successful biosensors. The literature in this field suggests that modification of electrodes with nanomaterials is an excellent method for enzyme immobilization, which can greatly improve the stability and sensitivity of the sensor. However, the poor stability, weak reproducibility, and limited lifetime of the enzyme itself still limit the requirements for the development of enzyme electrochemical biosensors for food production process monitoring. Therefore, constructing sensing technologies based on enzyme electrochemical biosensors remains a great challenge. This article outlines the construction principles of four generations of enzyme electrochemical biosensors and discusses the applications of single-enzyme systems, multi-enzyme systems, and nano-enzyme systems developed based on these principles. The article further describes methods to improve enzyme immobilization by combining different types of nanomaterials such as metals and their oxides, graphene-related materials, metal–organic frameworks, carbon nanotubes, and conducting polymers. In addition, the article highlights the challenges and future trends of enzyme electrochemical biosensors, providing theoretical support and future perspectives for further research and development of high-performance enzyme chemical biosensors. |
format | Online Article Text |
id | pubmed-10526424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105264242023-09-28 Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring Sun, Ganchao Wei, Xiaobo Zhang, Dianping Huang, Liben Liu, Huiyan Fang, Haitian Biosensors (Basel) Review Electrochemical biosensors based on immobilized enzymes are among the most popular and commercially successful biosensors. The literature in this field suggests that modification of electrodes with nanomaterials is an excellent method for enzyme immobilization, which can greatly improve the stability and sensitivity of the sensor. However, the poor stability, weak reproducibility, and limited lifetime of the enzyme itself still limit the requirements for the development of enzyme electrochemical biosensors for food production process monitoring. Therefore, constructing sensing technologies based on enzyme electrochemical biosensors remains a great challenge. This article outlines the construction principles of four generations of enzyme electrochemical biosensors and discusses the applications of single-enzyme systems, multi-enzyme systems, and nano-enzyme systems developed based on these principles. The article further describes methods to improve enzyme immobilization by combining different types of nanomaterials such as metals and their oxides, graphene-related materials, metal–organic frameworks, carbon nanotubes, and conducting polymers. In addition, the article highlights the challenges and future trends of enzyme electrochemical biosensors, providing theoretical support and future perspectives for further research and development of high-performance enzyme chemical biosensors. MDPI 2023-09-17 /pmc/articles/PMC10526424/ /pubmed/37754120 http://dx.doi.org/10.3390/bios13090886 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Sun, Ganchao Wei, Xiaobo Zhang, Dianping Huang, Liben Liu, Huiyan Fang, Haitian Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring |
title | Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring |
title_full | Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring |
title_fullStr | Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring |
title_full_unstemmed | Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring |
title_short | Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring |
title_sort | immobilization of enzyme electrochemical biosensors and their application to food bioprocess monitoring |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526424/ https://www.ncbi.nlm.nih.gov/pubmed/37754120 http://dx.doi.org/10.3390/bios13090886 |
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