Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance

Lateral flow tests are one of the most important types of paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving the rapid screening and management of infections in global pandemics or other potential health disorders by using minimally expert staff...

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Autores principales: Omidfar, Kobra, Riahi, Fatemeh, Kashanian, Soheila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526804/
https://www.ncbi.nlm.nih.gov/pubmed/37754072
http://dx.doi.org/10.3390/bios13090837
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author Omidfar, Kobra
Riahi, Fatemeh
Kashanian, Soheila
author_facet Omidfar, Kobra
Riahi, Fatemeh
Kashanian, Soheila
author_sort Omidfar, Kobra
collection PubMed
description Lateral flow tests are one of the most important types of paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving the rapid screening and management of infections in global pandemics or other potential health disorders by using minimally expert staff in locations where no sophisticated laboratory services are accessible. They can detect different types of biomarkers in various biological samples and provide the results in a little time at a low price. An important challenge regarding conventional LFAs is increasing their sensitivity and specificity. There are two main approaches to increase sensitivity and specificity, including assay improvement and target enrichment. Assay improvement comprises the assay optimization and signal amplification techniques. In this study, a summarize of various sensitivity and specificity enhancement strategies with an objective evaluation are presented, such as detection element immobilization, capillary flow rate adjusting, label evolution, sample extraction and enrichment, etc. and also the key findings in improving the LFA performance and solving their limitations are discussed along with numerous examples.
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spelling pubmed-105268042023-09-28 Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance Omidfar, Kobra Riahi, Fatemeh Kashanian, Soheila Biosensors (Basel) Review Lateral flow tests are one of the most important types of paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving the rapid screening and management of infections in global pandemics or other potential health disorders by using minimally expert staff in locations where no sophisticated laboratory services are accessible. They can detect different types of biomarkers in various biological samples and provide the results in a little time at a low price. An important challenge regarding conventional LFAs is increasing their sensitivity and specificity. There are two main approaches to increase sensitivity and specificity, including assay improvement and target enrichment. Assay improvement comprises the assay optimization and signal amplification techniques. In this study, a summarize of various sensitivity and specificity enhancement strategies with an objective evaluation are presented, such as detection element immobilization, capillary flow rate adjusting, label evolution, sample extraction and enrichment, etc. and also the key findings in improving the LFA performance and solving their limitations are discussed along with numerous examples. MDPI 2023-08-23 /pmc/articles/PMC10526804/ /pubmed/37754072 http://dx.doi.org/10.3390/bios13090837 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Omidfar, Kobra
Riahi, Fatemeh
Kashanian, Soheila
Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance
title Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance
title_full Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance
title_fullStr Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance
title_full_unstemmed Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance
title_short Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance
title_sort lateral flow assay: a summary of recent progress for improving assay performance
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526804/
https://www.ncbi.nlm.nih.gov/pubmed/37754072
http://dx.doi.org/10.3390/bios13090837
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