Cargando…
Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides
Dairy protein hydrolysates possess a broad spectrum of bioactivity and hypoallergenic properties, as well as pronounced bitter taste. The bitterness is reduced by complexing the proteolysis products with cyclodextrins (CDs), and it is also important to study the bioactivity of the peptides in inclus...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530839/ https://www.ncbi.nlm.nih.gov/pubmed/37762289 http://dx.doi.org/10.3390/ijms241813987 |
_version_ | 1785111579238334464 |
---|---|
author | Halavach, Tatsiana M. Kurchenko, Vladimir P. Tarun, Ekaterina I. Dudchik, Natalia V. Yatskou, Mikalai M. Lodygin, Aleksey D. Alieva, Ludmila R. Evdokimov, Ivan A. Ulrih, Natasa Poklar |
author_facet | Halavach, Tatsiana M. Kurchenko, Vladimir P. Tarun, Ekaterina I. Dudchik, Natalia V. Yatskou, Mikalai M. Lodygin, Aleksey D. Alieva, Ludmila R. Evdokimov, Ivan A. Ulrih, Natasa Poklar |
author_sort | Halavach, Tatsiana M. |
collection | PubMed |
description | Dairy protein hydrolysates possess a broad spectrum of bioactivity and hypoallergenic properties, as well as pronounced bitter taste. The bitterness is reduced by complexing the proteolysis products with cyclodextrins (CDs), and it is also important to study the bioactivity of the peptides in inclusion complexes. Hydrolysates of whey and colostrum proteins with extensive hydrolysis degree and their complexes with β/γ-CD were obtained in the present study, and comprehensive comparative analysis of the experimental samples was performed. The interaction of CD with peptides was confirmed via different methods. Bioactivity of the initial hydrolysates and their complexes were evaluated. Antioxidant activity (AOA) was determined by fluorescence reduction of fluorescein in the Fenton system. Antigenic properties were studied by competitive enzyme immunoassay. Antimutagenic effect was estimated in the Ames test. According to the experimental data, a 2.17/2.78-fold and 1.45/2.14-fold increase in the AOA was found in the β/γ-CD interaction with whey and colostrum hydrolysates, respectively. A 5.6/5.3-fold decrease in the antigenicity of whey peptides in complex with β/γ-CD was detected, while the antimutagenic effect in the host–guest systems was comparable to the initial hydrolysates. Thus, bioactive CD complexes with dairy peptides were obtained. Complexes are applicable as a component of specialized foods (sports, diet). |
format | Online Article Text |
id | pubmed-10530839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105308392023-09-28 Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides Halavach, Tatsiana M. Kurchenko, Vladimir P. Tarun, Ekaterina I. Dudchik, Natalia V. Yatskou, Mikalai M. Lodygin, Aleksey D. Alieva, Ludmila R. Evdokimov, Ivan A. Ulrih, Natasa Poklar Int J Mol Sci Article Dairy protein hydrolysates possess a broad spectrum of bioactivity and hypoallergenic properties, as well as pronounced bitter taste. The bitterness is reduced by complexing the proteolysis products with cyclodextrins (CDs), and it is also important to study the bioactivity of the peptides in inclusion complexes. Hydrolysates of whey and colostrum proteins with extensive hydrolysis degree and their complexes with β/γ-CD were obtained in the present study, and comprehensive comparative analysis of the experimental samples was performed. The interaction of CD with peptides was confirmed via different methods. Bioactivity of the initial hydrolysates and their complexes were evaluated. Antioxidant activity (AOA) was determined by fluorescence reduction of fluorescein in the Fenton system. Antigenic properties were studied by competitive enzyme immunoassay. Antimutagenic effect was estimated in the Ames test. According to the experimental data, a 2.17/2.78-fold and 1.45/2.14-fold increase in the AOA was found in the β/γ-CD interaction with whey and colostrum hydrolysates, respectively. A 5.6/5.3-fold decrease in the antigenicity of whey peptides in complex with β/γ-CD was detected, while the antimutagenic effect in the host–guest systems was comparable to the initial hydrolysates. Thus, bioactive CD complexes with dairy peptides were obtained. Complexes are applicable as a component of specialized foods (sports, diet). MDPI 2023-09-12 /pmc/articles/PMC10530839/ /pubmed/37762289 http://dx.doi.org/10.3390/ijms241813987 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Halavach, Tatsiana M. Kurchenko, Vladimir P. Tarun, Ekaterina I. Dudchik, Natalia V. Yatskou, Mikalai M. Lodygin, Aleksey D. Alieva, Ludmila R. Evdokimov, Ivan A. Ulrih, Natasa Poklar Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides |
title | Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides |
title_full | Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides |
title_fullStr | Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides |
title_full_unstemmed | Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides |
title_short | Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides |
title_sort | influence of complexation with β- and γ-cyclodextrin on bioactivity of whey and colostrum peptides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530839/ https://www.ncbi.nlm.nih.gov/pubmed/37762289 http://dx.doi.org/10.3390/ijms241813987 |
work_keys_str_mv | AT halavachtatsianam influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides AT kurchenkovladimirp influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides AT tarunekaterinai influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides AT dudchiknataliav influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides AT yatskoumikalaim influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides AT lodyginalekseyd influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides AT alievaludmilar influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides AT evdokimovivana influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides AT ulrihnatasapoklar influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides |