Cargando…

Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides

Dairy protein hydrolysates possess a broad spectrum of bioactivity and hypoallergenic properties, as well as pronounced bitter taste. The bitterness is reduced by complexing the proteolysis products with cyclodextrins (CDs), and it is also important to study the bioactivity of the peptides in inclus...

Descripción completa

Detalles Bibliográficos
Autores principales: Halavach, Tatsiana M., Kurchenko, Vladimir P., Tarun, Ekaterina I., Dudchik, Natalia V., Yatskou, Mikalai M., Lodygin, Aleksey D., Alieva, Ludmila R., Evdokimov, Ivan A., Ulrih, Natasa Poklar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530839/
https://www.ncbi.nlm.nih.gov/pubmed/37762289
http://dx.doi.org/10.3390/ijms241813987
_version_ 1785111579238334464
author Halavach, Tatsiana M.
Kurchenko, Vladimir P.
Tarun, Ekaterina I.
Dudchik, Natalia V.
Yatskou, Mikalai M.
Lodygin, Aleksey D.
Alieva, Ludmila R.
Evdokimov, Ivan A.
Ulrih, Natasa Poklar
author_facet Halavach, Tatsiana M.
Kurchenko, Vladimir P.
Tarun, Ekaterina I.
Dudchik, Natalia V.
Yatskou, Mikalai M.
Lodygin, Aleksey D.
Alieva, Ludmila R.
Evdokimov, Ivan A.
Ulrih, Natasa Poklar
author_sort Halavach, Tatsiana M.
collection PubMed
description Dairy protein hydrolysates possess a broad spectrum of bioactivity and hypoallergenic properties, as well as pronounced bitter taste. The bitterness is reduced by complexing the proteolysis products with cyclodextrins (CDs), and it is also important to study the bioactivity of the peptides in inclusion complexes. Hydrolysates of whey and colostrum proteins with extensive hydrolysis degree and their complexes with β/γ-CD were obtained in the present study, and comprehensive comparative analysis of the experimental samples was performed. The interaction of CD with peptides was confirmed via different methods. Bioactivity of the initial hydrolysates and their complexes were evaluated. Antioxidant activity (AOA) was determined by fluorescence reduction of fluorescein in the Fenton system. Antigenic properties were studied by competitive enzyme immunoassay. Antimutagenic effect was estimated in the Ames test. According to the experimental data, a 2.17/2.78-fold and 1.45/2.14-fold increase in the AOA was found in the β/γ-CD interaction with whey and colostrum hydrolysates, respectively. A 5.6/5.3-fold decrease in the antigenicity of whey peptides in complex with β/γ-CD was detected, while the antimutagenic effect in the host–guest systems was comparable to the initial hydrolysates. Thus, bioactive CD complexes with dairy peptides were obtained. Complexes are applicable as a component of specialized foods (sports, diet).
format Online
Article
Text
id pubmed-10530839
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105308392023-09-28 Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides Halavach, Tatsiana M. Kurchenko, Vladimir P. Tarun, Ekaterina I. Dudchik, Natalia V. Yatskou, Mikalai M. Lodygin, Aleksey D. Alieva, Ludmila R. Evdokimov, Ivan A. Ulrih, Natasa Poklar Int J Mol Sci Article Dairy protein hydrolysates possess a broad spectrum of bioactivity and hypoallergenic properties, as well as pronounced bitter taste. The bitterness is reduced by complexing the proteolysis products with cyclodextrins (CDs), and it is also important to study the bioactivity of the peptides in inclusion complexes. Hydrolysates of whey and colostrum proteins with extensive hydrolysis degree and their complexes with β/γ-CD were obtained in the present study, and comprehensive comparative analysis of the experimental samples was performed. The interaction of CD with peptides was confirmed via different methods. Bioactivity of the initial hydrolysates and their complexes were evaluated. Antioxidant activity (AOA) was determined by fluorescence reduction of fluorescein in the Fenton system. Antigenic properties were studied by competitive enzyme immunoassay. Antimutagenic effect was estimated in the Ames test. According to the experimental data, a 2.17/2.78-fold and 1.45/2.14-fold increase in the AOA was found in the β/γ-CD interaction with whey and colostrum hydrolysates, respectively. A 5.6/5.3-fold decrease in the antigenicity of whey peptides in complex with β/γ-CD was detected, while the antimutagenic effect in the host–guest systems was comparable to the initial hydrolysates. Thus, bioactive CD complexes with dairy peptides were obtained. Complexes are applicable as a component of specialized foods (sports, diet). MDPI 2023-09-12 /pmc/articles/PMC10530839/ /pubmed/37762289 http://dx.doi.org/10.3390/ijms241813987 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Halavach, Tatsiana M.
Kurchenko, Vladimir P.
Tarun, Ekaterina I.
Dudchik, Natalia V.
Yatskou, Mikalai M.
Lodygin, Aleksey D.
Alieva, Ludmila R.
Evdokimov, Ivan A.
Ulrih, Natasa Poklar
Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides
title Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides
title_full Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides
title_fullStr Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides
title_full_unstemmed Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides
title_short Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides
title_sort influence of complexation with β- and γ-cyclodextrin on bioactivity of whey and colostrum peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530839/
https://www.ncbi.nlm.nih.gov/pubmed/37762289
http://dx.doi.org/10.3390/ijms241813987
work_keys_str_mv AT halavachtatsianam influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides
AT kurchenkovladimirp influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides
AT tarunekaterinai influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides
AT dudchiknataliav influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides
AT yatskoumikalaim influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides
AT lodyginalekseyd influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides
AT alievaludmilar influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides
AT evdokimovivana influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides
AT ulrihnatasapoklar influenceofcomplexationwithbandgcyclodextrinonbioactivityofwheyandcolostrumpeptides