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Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway

Keloid scars are fibro-proliferative conditions characterized by abnormal fibroblast proliferation and excessive extracellular matrix deposition. The mammalian target of the rapamycin (mTOR) pathway has emerged as a potential therapeutic target in keloid disease. Silibinin, a natural flavonoid isola...

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Detalles Bibliográficos
Autores principales: Choi, Sooyeon, Ham, Seoyoon, Lee, Young In, Kim, Jihee, Lee, Won Jai, Lee, Ju Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531945/
https://www.ncbi.nlm.nih.gov/pubmed/37762688
http://dx.doi.org/10.3390/ijms241814386
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author Choi, Sooyeon
Ham, Seoyoon
Lee, Young In
Kim, Jihee
Lee, Won Jai
Lee, Ju Hee
author_facet Choi, Sooyeon
Ham, Seoyoon
Lee, Young In
Kim, Jihee
Lee, Won Jai
Lee, Ju Hee
author_sort Choi, Sooyeon
collection PubMed
description Keloid scars are fibro-proliferative conditions characterized by abnormal fibroblast proliferation and excessive extracellular matrix deposition. The mammalian target of the rapamycin (mTOR) pathway has emerged as a potential therapeutic target in keloid disease. Silibinin, a natural flavonoid isolated from the seeds and fruits of the milk thistle, is known to inhibit the mTOR signaling pathway in human cervical and hepatoma cancer cells. However, the mechanisms underlying this inhibitory effect are not fully understood. This in vitro study investigated the effects of silibinin on collagen expression in normal human dermal and keloid-derived fibroblasts. We evaluated the effects of silibinin on the expressions of collagen types I and III and assessed its effects on the suppression of the mTOR signaling pathway. Our findings confirmed elevated mTOR phosphorylation levels in keloid scars compared to normal tissue specimens. Silibinin treatment significantly reduced collagen I and III expressions in normal human dermal and keloid-derived fibroblasts. These effects were accompanied by the suppression of the mTOR signaling pathway. Our findings suggest the potential of silibinin as a promising therapeutic agent for preventing and treating keloid scars. Further studies are warranted to explore the clinical application of silibinin in scar management.
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spelling pubmed-105319452023-09-28 Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway Choi, Sooyeon Ham, Seoyoon Lee, Young In Kim, Jihee Lee, Won Jai Lee, Ju Hee Int J Mol Sci Article Keloid scars are fibro-proliferative conditions characterized by abnormal fibroblast proliferation and excessive extracellular matrix deposition. The mammalian target of the rapamycin (mTOR) pathway has emerged as a potential therapeutic target in keloid disease. Silibinin, a natural flavonoid isolated from the seeds and fruits of the milk thistle, is known to inhibit the mTOR signaling pathway in human cervical and hepatoma cancer cells. However, the mechanisms underlying this inhibitory effect are not fully understood. This in vitro study investigated the effects of silibinin on collagen expression in normal human dermal and keloid-derived fibroblasts. We evaluated the effects of silibinin on the expressions of collagen types I and III and assessed its effects on the suppression of the mTOR signaling pathway. Our findings confirmed elevated mTOR phosphorylation levels in keloid scars compared to normal tissue specimens. Silibinin treatment significantly reduced collagen I and III expressions in normal human dermal and keloid-derived fibroblasts. These effects were accompanied by the suppression of the mTOR signaling pathway. Our findings suggest the potential of silibinin as a promising therapeutic agent for preventing and treating keloid scars. Further studies are warranted to explore the clinical application of silibinin in scar management. MDPI 2023-09-21 /pmc/articles/PMC10531945/ /pubmed/37762688 http://dx.doi.org/10.3390/ijms241814386 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Choi, Sooyeon
Ham, Seoyoon
Lee, Young In
Kim, Jihee
Lee, Won Jai
Lee, Ju Hee
Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway
title Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway
title_full Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway
title_fullStr Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway
title_full_unstemmed Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway
title_short Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway
title_sort silibinin downregulates types i and iii collagen expression via suppression of the mtor signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531945/
https://www.ncbi.nlm.nih.gov/pubmed/37762688
http://dx.doi.org/10.3390/ijms241814386
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