Cargando…

Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed

Soybean (Glycine max (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathio...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yuchen, Wang, Qingyu, Liu, Yajing, Dong, Shuo, Zhang, Yongqiang, Zhu, Youcheng, Tian, Yu, Li, Jingwen, Wang, Zhuoyi, Wang, Ying, Yan, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532240/
https://www.ncbi.nlm.nih.gov/pubmed/37762432
http://dx.doi.org/10.3390/ijms241814125
_version_ 1785111909384585216
author Zhang, Yuchen
Wang, Qingyu
Liu, Yajing
Dong, Shuo
Zhang, Yongqiang
Zhu, Youcheng
Tian, Yu
Li, Jingwen
Wang, Zhuoyi
Wang, Ying
Yan, Fan
author_facet Zhang, Yuchen
Wang, Qingyu
Liu, Yajing
Dong, Shuo
Zhang, Yongqiang
Zhu, Youcheng
Tian, Yu
Li, Jingwen
Wang, Zhuoyi
Wang, Ying
Yan, Fan
author_sort Zhang, Yuchen
collection PubMed
description Soybean (Glycine max (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathionine γ-synthase 2 (GmCGS2), the first unique enzyme in Met biosynthesis, was overexpressed in the soybean cultivar “Jack”, producing three transgenic lines (OE3, OE4, and OE10). We detected a considerable increase in the content of free Met and other free amino acids in the developing seeds of the three transgenic lines at the 15th and 75th days after flowering (15D and 75D). In addition, transcriptome analysis showed that the expression of genes related to Met biosynthesis from the aspartate-family pathway and S-methyl Met cycle was promoted in developing green seeds of OE10. Ultimately, the accumulation of total amino acids and soluble proteins in transgenic mature seeds was promoted. Altogether, these results indicated that GmCGS2 plays an important role in Met biosynthesis, by providing a basis for improving the nutritional quality of soybean seeds.
format Online
Article
Text
id pubmed-10532240
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105322402023-09-28 Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed Zhang, Yuchen Wang, Qingyu Liu, Yajing Dong, Shuo Zhang, Yongqiang Zhu, Youcheng Tian, Yu Li, Jingwen Wang, Zhuoyi Wang, Ying Yan, Fan Int J Mol Sci Article Soybean (Glycine max (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathionine γ-synthase 2 (GmCGS2), the first unique enzyme in Met biosynthesis, was overexpressed in the soybean cultivar “Jack”, producing three transgenic lines (OE3, OE4, and OE10). We detected a considerable increase in the content of free Met and other free amino acids in the developing seeds of the three transgenic lines at the 15th and 75th days after flowering (15D and 75D). In addition, transcriptome analysis showed that the expression of genes related to Met biosynthesis from the aspartate-family pathway and S-methyl Met cycle was promoted in developing green seeds of OE10. Ultimately, the accumulation of total amino acids and soluble proteins in transgenic mature seeds was promoted. Altogether, these results indicated that GmCGS2 plays an important role in Met biosynthesis, by providing a basis for improving the nutritional quality of soybean seeds. MDPI 2023-09-15 /pmc/articles/PMC10532240/ /pubmed/37762432 http://dx.doi.org/10.3390/ijms241814125 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yuchen
Wang, Qingyu
Liu, Yajing
Dong, Shuo
Zhang, Yongqiang
Zhu, Youcheng
Tian, Yu
Li, Jingwen
Wang, Zhuoyi
Wang, Ying
Yan, Fan
Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed
title Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed
title_full Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed
title_fullStr Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed
title_full_unstemmed Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed
title_short Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed
title_sort overexpressing gmcgs2 improves total amino acid and protein content in soybean seed
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532240/
https://www.ncbi.nlm.nih.gov/pubmed/37762432
http://dx.doi.org/10.3390/ijms241814125
work_keys_str_mv AT zhangyuchen overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT wangqingyu overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT liuyajing overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT dongshuo overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT zhangyongqiang overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT zhuyoucheng overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT tianyu overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT lijingwen overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT wangzhuoyi overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT wangying overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed
AT yanfan overexpressinggmcgs2improvestotalaminoacidandproteincontentinsoybeanseed