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Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed
Soybean (Glycine max (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathio...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532240/ https://www.ncbi.nlm.nih.gov/pubmed/37762432 http://dx.doi.org/10.3390/ijms241814125 |
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author | Zhang, Yuchen Wang, Qingyu Liu, Yajing Dong, Shuo Zhang, Yongqiang Zhu, Youcheng Tian, Yu Li, Jingwen Wang, Zhuoyi Wang, Ying Yan, Fan |
author_facet | Zhang, Yuchen Wang, Qingyu Liu, Yajing Dong, Shuo Zhang, Yongqiang Zhu, Youcheng Tian, Yu Li, Jingwen Wang, Zhuoyi Wang, Ying Yan, Fan |
author_sort | Zhang, Yuchen |
collection | PubMed |
description | Soybean (Glycine max (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathionine γ-synthase 2 (GmCGS2), the first unique enzyme in Met biosynthesis, was overexpressed in the soybean cultivar “Jack”, producing three transgenic lines (OE3, OE4, and OE10). We detected a considerable increase in the content of free Met and other free amino acids in the developing seeds of the three transgenic lines at the 15th and 75th days after flowering (15D and 75D). In addition, transcriptome analysis showed that the expression of genes related to Met biosynthesis from the aspartate-family pathway and S-methyl Met cycle was promoted in developing green seeds of OE10. Ultimately, the accumulation of total amino acids and soluble proteins in transgenic mature seeds was promoted. Altogether, these results indicated that GmCGS2 plays an important role in Met biosynthesis, by providing a basis for improving the nutritional quality of soybean seeds. |
format | Online Article Text |
id | pubmed-10532240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105322402023-09-28 Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed Zhang, Yuchen Wang, Qingyu Liu, Yajing Dong, Shuo Zhang, Yongqiang Zhu, Youcheng Tian, Yu Li, Jingwen Wang, Zhuoyi Wang, Ying Yan, Fan Int J Mol Sci Article Soybean (Glycine max (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathionine γ-synthase 2 (GmCGS2), the first unique enzyme in Met biosynthesis, was overexpressed in the soybean cultivar “Jack”, producing three transgenic lines (OE3, OE4, and OE10). We detected a considerable increase in the content of free Met and other free amino acids in the developing seeds of the three transgenic lines at the 15th and 75th days after flowering (15D and 75D). In addition, transcriptome analysis showed that the expression of genes related to Met biosynthesis from the aspartate-family pathway and S-methyl Met cycle was promoted in developing green seeds of OE10. Ultimately, the accumulation of total amino acids and soluble proteins in transgenic mature seeds was promoted. Altogether, these results indicated that GmCGS2 plays an important role in Met biosynthesis, by providing a basis for improving the nutritional quality of soybean seeds. MDPI 2023-09-15 /pmc/articles/PMC10532240/ /pubmed/37762432 http://dx.doi.org/10.3390/ijms241814125 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Yuchen Wang, Qingyu Liu, Yajing Dong, Shuo Zhang, Yongqiang Zhu, Youcheng Tian, Yu Li, Jingwen Wang, Zhuoyi Wang, Ying Yan, Fan Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed |
title | Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed |
title_full | Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed |
title_fullStr | Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed |
title_full_unstemmed | Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed |
title_short | Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed |
title_sort | overexpressing gmcgs2 improves total amino acid and protein content in soybean seed |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532240/ https://www.ncbi.nlm.nih.gov/pubmed/37762432 http://dx.doi.org/10.3390/ijms241814125 |
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