A Method for Rapid Polyethyleneimine-Based Purification of Bacteriophage-Expressed Proteins from Diluted Crude Lysates, Exemplified by Thermostable TP-84 Depolymerase

Purification of bacteriophage-expressed proteins poses methodological difficulties associated with the need to process entire culture medium volume upon bacteriophage-induced bacterial cell lysis. We have used novel capsule glycosylase-depolymerase (TP84_26 GD) from bacteriophage TP-84, infecting th...

Descripción completa

Detalles Bibliográficos
Autores principales: Łubkowska, Beata, Czajkowska, Edyta, Sobolewski, Ireneusz, Krawczun, Natalia, Żylicz-Stachula, Agnieszka, Skowron, Piotr M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535936/
https://www.ncbi.nlm.nih.gov/pubmed/37764184
http://dx.doi.org/10.3390/microorganisms11092340

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535936/
https://www.ncbi.nlm.nih.gov/pubmed/37764184
http://dx.doi.org/10.3390/microorganisms11092340

Ejemplares similares