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A Method for Rapid Polyethyleneimine-Based Purification of Bacteriophage-Expressed Proteins from Diluted Crude Lysates, Exemplified by Thermostable TP-84 Depolymerase

Purification of bacteriophage-expressed proteins poses methodological difficulties associated with the need to process entire culture medium volume upon bacteriophage-induced bacterial cell lysis. We have used novel capsule glycosylase-depolymerase (TP84_26 GD) from bacteriophage TP-84, infecting th...

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Detalles Bibliográficos
Autores principales:	Łubkowska, Beata, Czajkowska, Edyta, Sobolewski, Ireneusz, Krawczun, Natalia, Żylicz-Stachula, Agnieszka, Skowron, Piotr M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535936/ https://www.ncbi.nlm.nih.gov/pubmed/37764184 http://dx.doi.org/10.3390/microorganisms11092340

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