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Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains

Infections with Mycoplasma gallisepticum (MG) in poultry are associated with a wide range of disease conditions, including those affecting the respiratory and reproductive systems. The purpose of this study was to endorse the more sensitive diagnostic scheme for MG infection and identify the best mo...

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Autores principales: Al-baqir, Ahmed, Hassanin, Ola, Al-Rasheed, Mohammed, Ahmed, Mohamed S., Mohamed, Mahmoud H. A., El Sayed, Mohamed Shawky, Megahed, Mohamed, El-Demerdash, Azza, Hashem, Youserya, Eid, Amal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536284/
https://www.ncbi.nlm.nih.gov/pubmed/37764939
http://dx.doi.org/10.3390/pathogens12091131
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author Al-baqir, Ahmed
Hassanin, Ola
Al-Rasheed, Mohammed
Ahmed, Mohamed S.
Mohamed, Mahmoud H. A.
El Sayed, Mohamed Shawky
Megahed, Mohamed
El-Demerdash, Azza
Hashem, Youserya
Eid, Amal
author_facet Al-baqir, Ahmed
Hassanin, Ola
Al-Rasheed, Mohammed
Ahmed, Mohamed S.
Mohamed, Mahmoud H. A.
El Sayed, Mohamed Shawky
Megahed, Mohamed
El-Demerdash, Azza
Hashem, Youserya
Eid, Amal
author_sort Al-baqir, Ahmed
collection PubMed
description Infections with Mycoplasma gallisepticum (MG) in poultry are associated with a wide range of disease conditions, including those affecting the respiratory and reproductive systems. The purpose of this study was to endorse the more sensitive diagnostic scheme for MG infection and identify the best molecular marker for MG phylogenetic analysis using six housekeeping genes: mgc2, mraW, atpG, ugpA, DUF31196, and lgT. For these purposes, 55 poultry flocks of different species were screened using either qRT-PCR or PCR techniques analogous to conventional culturing from non-cultured and cultured swabs on PPLO broth. The rate of MG positivity was the highest when using qRT-PCR from cultured broth (89.0%) and the lowest when using conventional culturing (34.5%). Compared to qRT-PCR from broth, statistical analysis using the Roc curve in MedCalc statistical software showed that the PCR schemes (qRT-PCR from swabs and PCR from swabs and broth) performed better than conventional culturing in terms of sensitivity, accuracy, and area under the curve (AUC), suggesting that they may be more reliable schemes. Further support was added by Cohen’s kappa test, showing moderate agreement between the molecular approaches. Among the six screened genes, mgc2 and mraW had the highest detection rates (69% and 65.4%, respectively). The comparative phylogenetic analysis revealed that mgc2 or atpG gene sequences distinguished MG isolates into different clades with high discriminatory power.
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spelling pubmed-105362842023-09-29 Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains Al-baqir, Ahmed Hassanin, Ola Al-Rasheed, Mohammed Ahmed, Mohamed S. Mohamed, Mahmoud H. A. El Sayed, Mohamed Shawky Megahed, Mohamed El-Demerdash, Azza Hashem, Youserya Eid, Amal Pathogens Article Infections with Mycoplasma gallisepticum (MG) in poultry are associated with a wide range of disease conditions, including those affecting the respiratory and reproductive systems. The purpose of this study was to endorse the more sensitive diagnostic scheme for MG infection and identify the best molecular marker for MG phylogenetic analysis using six housekeeping genes: mgc2, mraW, atpG, ugpA, DUF31196, and lgT. For these purposes, 55 poultry flocks of different species were screened using either qRT-PCR or PCR techniques analogous to conventional culturing from non-cultured and cultured swabs on PPLO broth. The rate of MG positivity was the highest when using qRT-PCR from cultured broth (89.0%) and the lowest when using conventional culturing (34.5%). Compared to qRT-PCR from broth, statistical analysis using the Roc curve in MedCalc statistical software showed that the PCR schemes (qRT-PCR from swabs and PCR from swabs and broth) performed better than conventional culturing in terms of sensitivity, accuracy, and area under the curve (AUC), suggesting that they may be more reliable schemes. Further support was added by Cohen’s kappa test, showing moderate agreement between the molecular approaches. Among the six screened genes, mgc2 and mraW had the highest detection rates (69% and 65.4%, respectively). The comparative phylogenetic analysis revealed that mgc2 or atpG gene sequences distinguished MG isolates into different clades with high discriminatory power. MDPI 2023-09-05 /pmc/articles/PMC10536284/ /pubmed/37764939 http://dx.doi.org/10.3390/pathogens12091131 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-baqir, Ahmed
Hassanin, Ola
Al-Rasheed, Mohammed
Ahmed, Mohamed S.
Mohamed, Mahmoud H. A.
El Sayed, Mohamed Shawky
Megahed, Mohamed
El-Demerdash, Azza
Hashem, Youserya
Eid, Amal
Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains
title Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains
title_full Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains
title_fullStr Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains
title_full_unstemmed Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains
title_short Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains
title_sort mycoplasmosis in poultry: an evaluation of diagnostic schemes and molecular analysis of egyptian mycoplasma gallisepticum strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536284/
https://www.ncbi.nlm.nih.gov/pubmed/37764939
http://dx.doi.org/10.3390/pathogens12091131
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