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Comparative Performance of Rapid Diagnostics for the Detection of T-2 and HT-2 Toxins in Oats
The contamination of oat crops by trichothecene mycotoxins, T-2 and HT-2 is an ongoing threat to our food safety. Within the industry, there are increasing concerns about the continued and growing presence of these mycotoxins occurring in oat crops due to climate change, farming practices and the ha...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537295/ https://www.ncbi.nlm.nih.gov/pubmed/37764433 http://dx.doi.org/10.3390/molecules28186657 |
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author | Meneely, Julie Greer, Brett Kolawole, Oluwatobi He, Qiqi Elliott, Christopher |
author_facet | Meneely, Julie Greer, Brett Kolawole, Oluwatobi He, Qiqi Elliott, Christopher |
author_sort | Meneely, Julie |
collection | PubMed |
description | The contamination of oat crops by trichothecene mycotoxins, T-2 and HT-2 is an ongoing threat to our food safety. Within the industry, there are increasing concerns about the continued and growing presence of these mycotoxins occurring in oat crops due to climate change, farming practices and the handling of crops post-harvest. To safeguard human health, monitoring these mycotoxins in foodstuffs is paramount to ensure human exposure is limited. To achieve this, effective testing regimes must be established within the industry, consisting not only of rapid, reliable, and accurate analytical methods but also efficient sampling strategies. Four commercial rapid diagnostic kits were assessed against liquid chromatography coupled to mass spectrometry and included three lateral flow devices and one enzyme-linked immunosorbent assay. One-way ANOVA showed a p-value of 0.45 indicating no significant difference between the methods assessed. Qualitative analysis revealed test kits 1, 2, 3, and 4 showed false negative/false positive rates of 1.1/2.2, 7.6/0, 2.2/0, and 6.5/0 percent, respectively. Test Kit 1, the Neogen Reveal(®) Q+ MAX for T-2/HT-2 Kit provided the most reliable, accurate and cost-effective results. Furthermore, its ease of use and no requirement for technical skill makes it applicable for on-site testing. |
format | Online Article Text |
id | pubmed-10537295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105372952023-09-29 Comparative Performance of Rapid Diagnostics for the Detection of T-2 and HT-2 Toxins in Oats Meneely, Julie Greer, Brett Kolawole, Oluwatobi He, Qiqi Elliott, Christopher Molecules Article The contamination of oat crops by trichothecene mycotoxins, T-2 and HT-2 is an ongoing threat to our food safety. Within the industry, there are increasing concerns about the continued and growing presence of these mycotoxins occurring in oat crops due to climate change, farming practices and the handling of crops post-harvest. To safeguard human health, monitoring these mycotoxins in foodstuffs is paramount to ensure human exposure is limited. To achieve this, effective testing regimes must be established within the industry, consisting not only of rapid, reliable, and accurate analytical methods but also efficient sampling strategies. Four commercial rapid diagnostic kits were assessed against liquid chromatography coupled to mass spectrometry and included three lateral flow devices and one enzyme-linked immunosorbent assay. One-way ANOVA showed a p-value of 0.45 indicating no significant difference between the methods assessed. Qualitative analysis revealed test kits 1, 2, 3, and 4 showed false negative/false positive rates of 1.1/2.2, 7.6/0, 2.2/0, and 6.5/0 percent, respectively. Test Kit 1, the Neogen Reveal(®) Q+ MAX for T-2/HT-2 Kit provided the most reliable, accurate and cost-effective results. Furthermore, its ease of use and no requirement for technical skill makes it applicable for on-site testing. MDPI 2023-09-16 /pmc/articles/PMC10537295/ /pubmed/37764433 http://dx.doi.org/10.3390/molecules28186657 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Meneely, Julie Greer, Brett Kolawole, Oluwatobi He, Qiqi Elliott, Christopher Comparative Performance of Rapid Diagnostics for the Detection of T-2 and HT-2 Toxins in Oats |
title | Comparative Performance of Rapid Diagnostics for the Detection of T-2 and HT-2 Toxins in Oats |
title_full | Comparative Performance of Rapid Diagnostics for the Detection of T-2 and HT-2 Toxins in Oats |
title_fullStr | Comparative Performance of Rapid Diagnostics for the Detection of T-2 and HT-2 Toxins in Oats |
title_full_unstemmed | Comparative Performance of Rapid Diagnostics for the Detection of T-2 and HT-2 Toxins in Oats |
title_short | Comparative Performance of Rapid Diagnostics for the Detection of T-2 and HT-2 Toxins in Oats |
title_sort | comparative performance of rapid diagnostics for the detection of t-2 and ht-2 toxins in oats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10537295/ https://www.ncbi.nlm.nih.gov/pubmed/37764433 http://dx.doi.org/10.3390/molecules28186657 |
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