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Native Semisynthesis of Isopeptide-Linked Substrates for Specificity Analysis of Deubiquitinases and Ubl Proteases
[Image: see text] Post-translational modifications with ubiquitin (Ub) and ubiquitin-like proteins (Ubls) are regulated by isopeptidases termed deubiquitinases (DUBs) and Ubl proteases. Here, we describe a mild chemical method for the preparation of fluorescence polarization substrates for these enz...
Autores principales: | Zhao, Zhou, O’Dea, Rachel, Wendrich, Kim, Kazi, Nafizul, Gersch, Malte |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10540217/ https://www.ncbi.nlm.nih.gov/pubmed/37712884 http://dx.doi.org/10.1021/jacs.3c04062 |
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