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Single-cell Herpes Simplex Virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics

Single-cell analyses of viral infections often reveal heterogeneity that is not detected by traditional population-level studies. This study applies drop-based microfluidics to investigate the dynamics of HSV-1 infection of neurons at the single-cell level. We used micron-scale Matrigel beads, terme...

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Autores principales: Fredrikson, Jacob P., Domanico, Luke F., Pratt, Shawna L., Loveday, Emma K., Taylor, Matthew P., Chang, Connie B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542126/
https://www.ncbi.nlm.nih.gov/pubmed/37790515
http://dx.doi.org/10.1101/2023.09.18.558333
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author Fredrikson, Jacob P.
Domanico, Luke F.
Pratt, Shawna L.
Loveday, Emma K.
Taylor, Matthew P.
Chang, Connie B.
author_facet Fredrikson, Jacob P.
Domanico, Luke F.
Pratt, Shawna L.
Loveday, Emma K.
Taylor, Matthew P.
Chang, Connie B.
author_sort Fredrikson, Jacob P.
collection PubMed
description Single-cell analyses of viral infections often reveal heterogeneity that is not detected by traditional population-level studies. This study applies drop-based microfluidics to investigate the dynamics of HSV-1 infection of neurons at the single-cell level. We used micron-scale Matrigel beads, termed microgels, to culture individual murine Superior Cervical ganglia (SCG) neurons or epithelial cells. Microgel-cultured cells are subsequently enclosed in individual media-in-oil droplets with a dual fluorescent-reporter HSV-1, enabling real-time observation of viral gene expression and replication. Infection within drops revealed that the kinetics of initial viral gene expression and replication were dependent on the inoculating dose. Notably, increasing inoculating doses led to earlier onset of viral gene expression and more frequent productive viral replication. These observations provide crucial insights into the complexity of HSV-1 infection in neurons and emphasize the importance of studying single-cell outcomes of viral infection. The innovative techniques presented here for cell culture and infection in drops provide a foundation for future virology and neurobiology investigations.
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spelling pubmed-105421262023-10-03 Single-cell Herpes Simplex Virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics Fredrikson, Jacob P. Domanico, Luke F. Pratt, Shawna L. Loveday, Emma K. Taylor, Matthew P. Chang, Connie B. bioRxiv Article Single-cell analyses of viral infections often reveal heterogeneity that is not detected by traditional population-level studies. This study applies drop-based microfluidics to investigate the dynamics of HSV-1 infection of neurons at the single-cell level. We used micron-scale Matrigel beads, termed microgels, to culture individual murine Superior Cervical ganglia (SCG) neurons or epithelial cells. Microgel-cultured cells are subsequently enclosed in individual media-in-oil droplets with a dual fluorescent-reporter HSV-1, enabling real-time observation of viral gene expression and replication. Infection within drops revealed that the kinetics of initial viral gene expression and replication were dependent on the inoculating dose. Notably, increasing inoculating doses led to earlier onset of viral gene expression and more frequent productive viral replication. These observations provide crucial insights into the complexity of HSV-1 infection in neurons and emphasize the importance of studying single-cell outcomes of viral infection. The innovative techniques presented here for cell culture and infection in drops provide a foundation for future virology and neurobiology investigations. Cold Spring Harbor Laboratory 2023-09-18 /pmc/articles/PMC10542126/ /pubmed/37790515 http://dx.doi.org/10.1101/2023.09.18.558333 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Fredrikson, Jacob P.
Domanico, Luke F.
Pratt, Shawna L.
Loveday, Emma K.
Taylor, Matthew P.
Chang, Connie B.
Single-cell Herpes Simplex Virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics
title Single-cell Herpes Simplex Virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics
title_full Single-cell Herpes Simplex Virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics
title_fullStr Single-cell Herpes Simplex Virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics
title_full_unstemmed Single-cell Herpes Simplex Virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics
title_short Single-cell Herpes Simplex Virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics
title_sort single-cell herpes simplex virus type-1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542126/
https://www.ncbi.nlm.nih.gov/pubmed/37790515
http://dx.doi.org/10.1101/2023.09.18.558333
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