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A tool to automatically design multiplex PCR primer pairs for specific targets using diverse templates

Multiplex PCR is an increasingly popular method for identifying species, investigating environmental diversity, and conducting phylogenetic analysis. The complexity and increasing availability of diverse templates necessitate a highly automated approach to design degenerate primer pairs for specific...

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Detalles Bibliográficos
Autores principales: Yang, Lin, Ding, Feng, Lin, Qiang, Xie, Junhua, Fan, Wei, Dai, Fangyin, Cui, Peng, Liu, Wanfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542359/
https://www.ncbi.nlm.nih.gov/pubmed/37777580
http://dx.doi.org/10.1038/s41598-023-43825-0