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A tool to automatically design multiplex PCR primer pairs for specific targets using diverse templates
Multiplex PCR is an increasingly popular method for identifying species, investigating environmental diversity, and conducting phylogenetic analysis. The complexity and increasing availability of diverse templates necessitate a highly automated approach to design degenerate primer pairs for specific...
Autores principales: | Yang, Lin, Ding, Feng, Lin, Qiang, Xie, Junhua, Fan, Wei, Dai, Fangyin, Cui, Peng, Liu, Wanfei |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542359/ https://www.ncbi.nlm.nih.gov/pubmed/37777580 http://dx.doi.org/10.1038/s41598-023-43825-0 |
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