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Cell-cycle arrest at the G1/S boundary enhances transient voltage-gated ion channel expression in human and insect cells

Heterologous expression of recombinant ion channel subunits in cell lines is often limited by the presence of a low number of channels at the cell surface level. Here, we introduce a combination of two techniques: viral expression using the baculovirus system plus cell-cycle arrest at the G1/S bound...

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Detalles Bibliográficos
Autores principales: Eltokhi, Ahmed, Catterall, William A., Gamal El-Din, Tamer M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545908/
https://www.ncbi.nlm.nih.gov/pubmed/37751687
http://dx.doi.org/10.1016/j.crmeth.2023.100559