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Cell-cycle arrest at the G1/S boundary enhances transient voltage-gated ion channel expression in human and insect cells
Heterologous expression of recombinant ion channel subunits in cell lines is often limited by the presence of a low number of channels at the cell surface level. Here, we introduce a combination of two techniques: viral expression using the baculovirus system plus cell-cycle arrest at the G1/S bound...
Autores principales: | Eltokhi, Ahmed, Catterall, William A., Gamal El-Din, Tamer M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545908/ https://www.ncbi.nlm.nih.gov/pubmed/37751687 http://dx.doi.org/10.1016/j.crmeth.2023.100559 |
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