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DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough
The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556565/ https://www.ncbi.nlm.nih.gov/pubmed/37748060 http://dx.doi.org/10.1073/pnas.2308260120 |
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author | Hiramatsu, Yukihiro Nishida, Takashi Ota, Natsuko Tamaki, Yuki Nugraha, Dendi K. Horiguchi, Yasuhiko |
author_facet | Hiramatsu, Yukihiro Nishida, Takashi Ota, Natsuko Tamaki, Yuki Nugraha, Dendi K. Horiguchi, Yasuhiko |
author_sort | Hiramatsu, Yukihiro |
collection | PubMed |
description | The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of the G(i) family through ADP-ribosylation of their α subunits, causes coughing in combination with Vag8 and lipid A in B. pertussis infection. In contrast, the mechanism of cough induced by B. parapertussis, which produces Vag8 and lipopolysaccharide (LPS) containing lipid A, but not PTx, remained to be elucidated. Here, we show that a toxin we named deacylating autotransporter toxin (DAT) of B. parapertussis inactivates heterotrimeric G(i) GTPases through demyristoylation of their α subunits and contributes to cough production along with Vag8 and LPS. These results indicate that DAT plays a role in B. parapertussis infection in place of PTx. |
format | Online Article Text |
id | pubmed-10556565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-105565652023-10-07 DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough Hiramatsu, Yukihiro Nishida, Takashi Ota, Natsuko Tamaki, Yuki Nugraha, Dendi K. Horiguchi, Yasuhiko Proc Natl Acad Sci U S A Biological Sciences The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of the G(i) family through ADP-ribosylation of their α subunits, causes coughing in combination with Vag8 and lipid A in B. pertussis infection. In contrast, the mechanism of cough induced by B. parapertussis, which produces Vag8 and lipopolysaccharide (LPS) containing lipid A, but not PTx, remained to be elucidated. Here, we show that a toxin we named deacylating autotransporter toxin (DAT) of B. parapertussis inactivates heterotrimeric G(i) GTPases through demyristoylation of their α subunits and contributes to cough production along with Vag8 and LPS. These results indicate that DAT plays a role in B. parapertussis infection in place of PTx. National Academy of Sciences 2023-09-25 2023-10-03 /pmc/articles/PMC10556565/ /pubmed/37748060 http://dx.doi.org/10.1073/pnas.2308260120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Biological Sciences Hiramatsu, Yukihiro Nishida, Takashi Ota, Natsuko Tamaki, Yuki Nugraha, Dendi K. Horiguchi, Yasuhiko DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough |
title | DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough |
title_full | DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough |
title_fullStr | DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough |
title_full_unstemmed | DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough |
title_short | DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough |
title_sort | dat (deacylating autotransporter toxin) from bordetella parapertussis demyristoylates gα(i) gtpases and contributes to cough |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556565/ https://www.ncbi.nlm.nih.gov/pubmed/37748060 http://dx.doi.org/10.1073/pnas.2308260120 |
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