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DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough

The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of...

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Autores principales: Hiramatsu, Yukihiro, Nishida, Takashi, Ota, Natsuko, Tamaki, Yuki, Nugraha, Dendi K., Horiguchi, Yasuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556565/
https://www.ncbi.nlm.nih.gov/pubmed/37748060
http://dx.doi.org/10.1073/pnas.2308260120
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author Hiramatsu, Yukihiro
Nishida, Takashi
Ota, Natsuko
Tamaki, Yuki
Nugraha, Dendi K.
Horiguchi, Yasuhiko
author_facet Hiramatsu, Yukihiro
Nishida, Takashi
Ota, Natsuko
Tamaki, Yuki
Nugraha, Dendi K.
Horiguchi, Yasuhiko
author_sort Hiramatsu, Yukihiro
collection PubMed
description The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of the G(i) family through ADP-ribosylation of their α subunits, causes coughing in combination with Vag8 and lipid A in B. pertussis infection. In contrast, the mechanism of cough induced by B. parapertussis, which produces Vag8 and lipopolysaccharide (LPS) containing lipid A, but not PTx, remained to be elucidated. Here, we show that a toxin we named deacylating autotransporter toxin (DAT) of B. parapertussis inactivates heterotrimeric G(i) GTPases through demyristoylation of their α subunits and contributes to cough production along with Vag8 and LPS. These results indicate that DAT plays a role in B. parapertussis infection in place of PTx.
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spelling pubmed-105565652023-10-07 DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough Hiramatsu, Yukihiro Nishida, Takashi Ota, Natsuko Tamaki, Yuki Nugraha, Dendi K. Horiguchi, Yasuhiko Proc Natl Acad Sci U S A Biological Sciences The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of the G(i) family through ADP-ribosylation of their α subunits, causes coughing in combination with Vag8 and lipid A in B. pertussis infection. In contrast, the mechanism of cough induced by B. parapertussis, which produces Vag8 and lipopolysaccharide (LPS) containing lipid A, but not PTx, remained to be elucidated. Here, we show that a toxin we named deacylating autotransporter toxin (DAT) of B. parapertussis inactivates heterotrimeric G(i) GTPases through demyristoylation of their α subunits and contributes to cough production along with Vag8 and LPS. These results indicate that DAT plays a role in B. parapertussis infection in place of PTx. National Academy of Sciences 2023-09-25 2023-10-03 /pmc/articles/PMC10556565/ /pubmed/37748060 http://dx.doi.org/10.1073/pnas.2308260120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biological Sciences
Hiramatsu, Yukihiro
Nishida, Takashi
Ota, Natsuko
Tamaki, Yuki
Nugraha, Dendi K.
Horiguchi, Yasuhiko
DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough
title DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough
title_full DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough
title_fullStr DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough
title_full_unstemmed DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough
title_short DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα(i) GTPases and contributes to cough
title_sort dat (deacylating autotransporter toxin) from bordetella parapertussis demyristoylates gα(i) gtpases and contributes to cough
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556565/
https://www.ncbi.nlm.nih.gov/pubmed/37748060
http://dx.doi.org/10.1073/pnas.2308260120
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