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The key role of glutamine for protein expression and isotopic labeling in insect cells

Nuclear magnetic resonance studies of many physiologically important proteins have long been impeded by the necessity to express such proteins in isotope-labeled form in higher eukaryotic cells and the concomitant high costs of providing isotope-labeled amino acids in the growth medium. Economical r...

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Detalles Bibliográficos
Autores principales: Wu, Feng-Jie, Kronenberg, Domenic, Hertel, Ines, Grzesiek, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556780/
https://www.ncbi.nlm.nih.gov/pubmed/37553040
http://dx.doi.org/10.1016/j.jbc.2023.105142