Calorimetric analysis using DNA thermal stability to determine protein concentration

It was recently reported for two globular proteins and a short DNA hairpin in NaCl buffer that values of the transition heat capacities, C(p,DNA) and C(p,PRO), for equal concentrations (mg/mL) of DNA and proteins, are essentially equivalent (differ by less than 1%). Additional evidence for this equi...

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Detalles Bibliográficos
Autores principales: Eskew, Matthew W., Reardon, Patrick W., Benight, Albert S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557601/
https://www.ncbi.nlm.nih.gov/pubmed/37808849
http://dx.doi.org/10.1101/2023.09.25.559360

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557601/
https://www.ncbi.nlm.nih.gov/pubmed/37808849
http://dx.doi.org/10.1101/2023.09.25.559360

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