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Calorimetric analysis using DNA thermal stability to determine protein concentration
It was recently reported for two globular proteins and a short DNA hairpin in NaCl buffer that values of the transition heat capacities, C(p,DNA) and C(p,PRO), for equal concentrations (mg/mL) of DNA and proteins, are essentially equivalent (differ by less than 1%). Additional evidence for this equi...
Autores principales: | Eskew, Matthew W., Reardon, Patrick W., Benight, Albert S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557601/ https://www.ncbi.nlm.nih.gov/pubmed/37808849 http://dx.doi.org/10.1101/2023.09.25.559360 |
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