A Scalable Staining Strategy for Whole-Brain Connectomics

Mapping the complete synaptic connectivity of a mammalian brain would be transformative, revealing the pathways underlying perception, behavior, and memory. Serial section electron microscopy, via membrane staining using osmium tetroxide, is ideal for visualizing cells and synaptic connections but,...

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Detalles Bibliográficos
Autores principales: Lu, Xiaotang, Wu, Yuelong, Schalek, Richard L., Meirovitch, Yaron, Berger, Daniel R., Lichtman, Jeff W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557665/
https://www.ncbi.nlm.nih.gov/pubmed/37808722
http://dx.doi.org/10.1101/2023.09.26.558265
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author Lu, Xiaotang
Wu, Yuelong
Schalek, Richard L.
Meirovitch, Yaron
Berger, Daniel R.
Lichtman, Jeff W.
author_facet Lu, Xiaotang
Wu, Yuelong
Schalek, Richard L.
Meirovitch, Yaron
Berger, Daniel R.
Lichtman, Jeff W.
author_sort Lu, Xiaotang
collection PubMed
description Mapping the complete synaptic connectivity of a mammalian brain would be transformative, revealing the pathways underlying perception, behavior, and memory. Serial section electron microscopy, via membrane staining using osmium tetroxide, is ideal for visualizing cells and synaptic connections but, in whole brain samples, faces significant challenges related to chemical treatment and volume changes. These issues can adversely affect both the ultrastructural quality and macroscopic tissue integrity. By leveraging time-lapse X-ray imaging and brain proxies, we have developed a 12-step protocol, ODeCO, that effectively infiltrates osmium throughout an entire mouse brain while preserving ultrastructure without any cracks or fragmentation, a necessary prerequisite for constructing the first comprehensive mouse brain connectome.
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spelling pubmed-105576652023-10-07 A Scalable Staining Strategy for Whole-Brain Connectomics Lu, Xiaotang Wu, Yuelong Schalek, Richard L. Meirovitch, Yaron Berger, Daniel R. Lichtman, Jeff W. bioRxiv Article Mapping the complete synaptic connectivity of a mammalian brain would be transformative, revealing the pathways underlying perception, behavior, and memory. Serial section electron microscopy, via membrane staining using osmium tetroxide, is ideal for visualizing cells and synaptic connections but, in whole brain samples, faces significant challenges related to chemical treatment and volume changes. These issues can adversely affect both the ultrastructural quality and macroscopic tissue integrity. By leveraging time-lapse X-ray imaging and brain proxies, we have developed a 12-step protocol, ODeCO, that effectively infiltrates osmium throughout an entire mouse brain while preserving ultrastructure without any cracks or fragmentation, a necessary prerequisite for constructing the first comprehensive mouse brain connectome. Cold Spring Harbor Laboratory 2023-09-27 /pmc/articles/PMC10557665/ /pubmed/37808722 http://dx.doi.org/10.1101/2023.09.26.558265 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Lu, Xiaotang
Wu, Yuelong
Schalek, Richard L.
Meirovitch, Yaron
Berger, Daniel R.
Lichtman, Jeff W.
A Scalable Staining Strategy for Whole-Brain Connectomics
title A Scalable Staining Strategy for Whole-Brain Connectomics
title_full A Scalable Staining Strategy for Whole-Brain Connectomics
title_fullStr A Scalable Staining Strategy for Whole-Brain Connectomics
title_full_unstemmed A Scalable Staining Strategy for Whole-Brain Connectomics
title_short A Scalable Staining Strategy for Whole-Brain Connectomics
title_sort scalable staining strategy for whole-brain connectomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557665/
https://www.ncbi.nlm.nih.gov/pubmed/37808722
http://dx.doi.org/10.1101/2023.09.26.558265
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