Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites

Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in o...

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Detalles Bibliográficos
Autores principales: Atkinson, Laura, Lee, Jack CD., Lennon, Alexander, Shah, Divya, Storey, Nathaniel, Morfopoulou, Sofia, Harris, Kathryn A., Breuer, Judy, Brown, Julianne R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559231/
https://www.ncbi.nlm.nih.gov/pubmed/37809666
http://dx.doi.org/10.1016/j.heliyon.2023.e19854

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559231/
https://www.ncbi.nlm.nih.gov/pubmed/37809666
http://dx.doi.org/10.1016/j.heliyon.2023.e19854

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