Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites

Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in o...

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Detalles Bibliográficos
Autores principales: Atkinson, Laura, Lee, Jack CD., Lennon, Alexander, Shah, Divya, Storey, Nathaniel, Morfopoulou, Sofia, Harris, Kathryn A., Breuer, Judy, Brown, Julianne R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559231/
https://www.ncbi.nlm.nih.gov/pubmed/37809666
http://dx.doi.org/10.1016/j.heliyon.2023.e19854
Descripción
Sumario:Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in our clinical service, processing 178 specimens over the past 2.5 years in a laboratory that adheres to ISO:15189 standards. We have successfully used this protocol to diagnose multiple cases of encephalitis and hepatitis.

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