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Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites
Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in o...
Autores principales: | Atkinson, Laura, Lee, Jack CD., Lennon, Alexander, Shah, Divya, Storey, Nathaniel, Morfopoulou, Sofia, Harris, Kathryn A., Breuer, Judy, Brown, Julianne R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559231/ https://www.ncbi.nlm.nih.gov/pubmed/37809666 http://dx.doi.org/10.1016/j.heliyon.2023.e19854 |
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