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Expression and characterization of a protease-resistant β-d-fructofuranosidase BbFFase9 gene suitable for preparing invert sugars from soybean meal

A novel gene (BbFFase9), with an ORF of 1557 bp that encodes β-d-fructofuranosidase from Bifidobacteriaceae bacterium, was cloned and expressed in Escherichia coli. The recombinant protein (BbFFase9) was successfully purified and showed a single band with a molecular mass of 66.2 kDa. This was confi...

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Detalles Bibliográficos
Autores principales: Chen, Zhou, Shen, Yimei, Wang, Run, Li, Siting, Jia, Yingmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559283/
https://www.ncbi.nlm.nih.gov/pubmed/37809427
http://dx.doi.org/10.1016/j.heliyon.2023.e19889