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A robust and reliable methodology to perform GECI-based multi-time point neuronal calcium imaging within mixed cultures of human iPSC-derived cortical neurons

INTRODUCTION: Human induced pluripotent stem cells (iPSCs), with their ability to generate human neural cells (astrocytes and neurons) from patients, hold great promise for understanding the pathophysiology of major neuropsychiatric diseases such as schizophrenia and bipolar disorders, which include...

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Detalles Bibliográficos
Autores principales: Patel, Niraj, Ouellet, Vincent, Paquet-Mercier, François, Chetoui, Nizar, Bélanger, Erik, Paquet, Marie-Eve, Godin, Antoine G., Marquet, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560759/
https://www.ncbi.nlm.nih.gov/pubmed/37817802
http://dx.doi.org/10.3389/fnins.2023.1247397