A novel metric to improve mismatched primer selection and quantification accuracy in amplifying DNA repeats for quantitative polymerase chain reactions

In quantitative polymerase chain reaction (qPCR) experiments, primers containing mismatches with respect to the template are widely used in measuring repetitive DNA elements. Primer-template mismatches may lead to underestimation of the input sample quantity due to inefficient annealing and amplific...

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Detalles Bibliográficos
Autores principales: Xu, Eugenia Y., Schneper, Lisa M., Notterman, Daniel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561853/
https://www.ncbi.nlm.nih.gov/pubmed/37812635
http://dx.doi.org/10.1371/journal.pone.0292559

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561853/
https://www.ncbi.nlm.nih.gov/pubmed/37812635
http://dx.doi.org/10.1371/journal.pone.0292559

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