Cargando…

A novel metric to improve mismatched primer selection and quantification accuracy in amplifying DNA repeats for quantitative polymerase chain reactions

In quantitative polymerase chain reaction (qPCR) experiments, primers containing mismatches with respect to the template are widely used in measuring repetitive DNA elements. Primer-template mismatches may lead to underestimation of the input sample quantity due to inefficient annealing and amplific...

Descripción completa

Detalles Bibliográficos
Autores principales:	Xu, Eugenia Y., Schneper, Lisa M., Notterman, Daniel A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561853/ https://www.ncbi.nlm.nih.gov/pubmed/37812635 http://dx.doi.org/10.1371/journal.pone.0292559

Ejemplares similares

Deconstructing the Polymerase Chain Reaction: Understanding and Correcting Bias Associated with Primer Degeneracies and Primer-Template Mismatches
por: Green, Stefan J., et al.
Publicado: (2015)

Quantification of Antisense Oligonucleotides by Splint Ligation and Quantitative Polymerase Chain Reaction
por: Shin, Minwook, et al.
Publicado: (2022)

AmoA-Targeted Polymerase Chain Reaction Primers for the Specific Detection and Quantification of Comammox Nitrospira in the Environment
por: Pjevac, Petra, et al.
Publicado: (2017)

Selection for 3′-End Triplets for Polymerase Chain Reaction Primers
por: Onodera, Kenji
Publicado: (2007)

Selection for 3′ end triplets for polymerase chain reaction primers
por: Onodera, Kenji, et al.
Publicado: (2004)

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
por: Ye, Jian, et al.
Publicado: (2012)

NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions
por: Kechin, Andrey, et al.
Publicado: (2020)

A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance
por: Spandidos, Athanasia, et al.
Publicado: (2008)

Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens
por: Decat, Ellen, et al.
Publicado: (2012)

PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers
por: Walters, William A., et al.
Publicado: (2011)

NEW PRIMERS FOR DETECTION OF Leishmania infantumUSING POLYMERASE CHAIN REACTION
por: GUALDA, Kézia Peres, et al.
Publicado: (2015)

Polymerase Chain Reaction with Confronting Two‐pair Primers for Polymorphism Genotyping
por: Hamajima, Nobuyuki, et al.
Publicado: (2000)

Quantitative Reverse Transcriptase Polymerase Chain Reaction
por: Gommersall, Lyndon M., et al.
Publicado: (2007)

Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction
por: Sugawara, Isamu, et al.
Publicado: (1992)

Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
por: Oka, Tomoichiro, et al.
Publicado: (2020)

Understanding Primers and Polymerase Chain Reaction by Using a Kinesthetic Paper Model
por: Heineman, Richard H.
Publicado: (2022)

Robust Quantification of Polymerase Chain Reactions Using Global Fitting
por: Carr, Ana C., et al.
Publicado: (2012)

Quantification of minimal disseminated disease by quantitative polymerase chain reaction and digital polymerase chain reaction for NPM-ALK as a prognostic factor in children with anaplastic large cell lymphoma
por: Damm-Welk, Christine, et al.
Publicado: (2020)

Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
por: Sharma, Pooja, et al.
Publicado: (2010)

Graphene oxide enhances the specificity of the polymerase chain reaction by modifying primer-template matching
por: Wang, Yuanyuan, et al.
Publicado: (2017)

Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy
por: Faron, Gilles, et al.
Publicado: (2017)

Validation of Droplet Digital Polymerase Chain Reaction for Salmonella spp. Quantification
por: Villamil, Carolina, et al.
Publicado: (2020)

Designing primers and evaluation of the efficiency of propidium monoazide – Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius
por: Lai, Chieh-Hsien, et al.
Publicado: (2016)

Quantitative multiplexing analysis of PCR-amplified ribosomal RNA genes by hierarchical oligonucleotide primer extension reaction
por: Wu, Jer-Horng, et al.
Publicado: (2007)

Enzyme Treatment-Free and Ligation-Independent Cloning Using Caged Primers in Polymerase Chain Reactions
por: Kuzuya, Akinori, et al.
Publicado: (2011)

Deconstructing the Polymerase Chain Reaction II: an improved workflow and effects on artifact formation and primer degeneracy
por: Naqib, Ankur, et al.
Publicado: (2019)

Ratio of the Primers Used in Polymerase Chain Reaction-Stop Analysis Impacts the Resultant Banding Pattern
por: Xiang, Lijun, et al.
Publicado: (2023)

Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 Patients
por: Lawrence Panchali, Merlin Jayalal, et al.
Publicado: (2022)

Quantitative polymerase chain reaction analysis by deconvolution of internal standard
por: Hirakawa, Yasuko, et al.
Publicado: (2010)

Stepwise kinetic equilibrium models of quantitative polymerase chain reaction
por: Cobbs, Gary
Publicado: (2012)

Plasmid Copy Number Determination by Quantitative Polymerase Chain Reaction
por: Anindyajati,, et al.
Publicado: (2016)

Accurate Bulk Quantitation of Droplet Digital Polymerase Chain Reaction
por: Sun, Chen, et al.
Publicado: (2021)

Hydrogel capsule-based digital quantitative polymerase chain reaction
por: Tan, Zheng Lin, et al.
Publicado: (2023)

Quantitative Polymerase Chain Reaction System for Alongshan Virus Detection
por: Litov, Alexander G., et al.
Publicado: (2023)

Reference gene validation for the relative quantification of cannabinoid receptor expression in human odontoblasts via quantitative polymerase chain reaction
por: Navarro-Saiz, Laura M., et al.
Publicado: (2022)

Qualitative polymerase chain reaction versus quantitative polymerase chain reaction for the detection of minimal residual disease in children with acute lymphoblastic leukemia()
por: Scrideli, Carlos Alberto, et al.
Publicado: (2015)

A new reverse transcription-polymerase chain reaction method for accurate quantification
por: Shiao, Yih-Horng
Publicado: (2003)

Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms
por: Gerdes, Lars, et al.
Publicado: (2016)

Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)
por: Fukui, Kenji, et al.
Publicado: (2013)

POLYMERASE CHAIN REACTION
por: Wages, J.M.
Publicado: (2005)

Cannot write session to /tmp/vufind_sessions/sess_th1ceck641dra8ectbjgje4tla