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Dispensable Role of Aire in CD11c(+) Conventional Dendritic Cells for Antigen Presentation and Shaping the Transcriptome
Aire, the defect of which is responsible for the development of autoimmunity, is predominantly expressed in medullary thymic epithelial cells, and it controls a wide variety of genes, including those of tissue-restricted Ags, for establishing thymic tolerance. Aire is also expressed from APCs in the...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563386/ https://www.ncbi.nlm.nih.gov/pubmed/36695731 http://dx.doi.org/10.4049/immunohorizons.2200103 |
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author | Miyazawa, Ryuichiro Nagao, Jun-ichi Arita-Morioka, Ken-ichi Matsumoto, Minoru Morimoto, Junko Yoshida, Masaki Oya, Takeshi Tsuneyama, Koichi Yoshida, Hideyuki Tanaka, Yoshihiko Matsumoto, Mitsuru |
author_facet | Miyazawa, Ryuichiro Nagao, Jun-ichi Arita-Morioka, Ken-ichi Matsumoto, Minoru Morimoto, Junko Yoshida, Masaki Oya, Takeshi Tsuneyama, Koichi Yoshida, Hideyuki Tanaka, Yoshihiko Matsumoto, Mitsuru |
author_sort | Miyazawa, Ryuichiro |
collection | PubMed |
description | Aire, the defect of which is responsible for the development of autoimmunity, is predominantly expressed in medullary thymic epithelial cells, and it controls a wide variety of genes, including those of tissue-restricted Ags, for establishing thymic tolerance. Aire is also expressed from APCs in the periphery, called extrathymic Aire-expressing cells (eTACs), and their complementing role to thymic tolerance has been suggested. eTACs are composed of two distinct classes of APCs, conventional dendritic cell (cDC)–type and group 3 innate lymphoid cell (ILC3)-like–type expressing retinoic acid receptor–related orphan receptor γt (RORγt). Although the essential role of Aire in the latter in the Th17-mediated immune response against Candida albicans has been reported, the role of Aire in the cDC-type eTACs for this action has not been examined. Furthermore, the significance of Aire in the production of the transcriptome of the cDC-type eTACs remains unknown. We have approached these issues using a high-fidelity Aire-reporter mouse strain. We found that although the cDC-type eTACs dominated ILC3-like–type eTACs in number and they served as efficient APCs for the immune response against an exogenous Ag as well as for the C. albicans–specific Th17 immune response, loss of Aire in cDC-type eTACs showed no clear effect on these functions. Furthermore, loss of Aire showed no major impact on the transcriptome from cDC-type eTACs. These results suggested that Aire in cDC-type eTACs may not have a cell-intrinsic role in the immune response in contrast to the role of Aire in ILC3-like–type eTACs. |
format | Online Article Text |
id | pubmed-10563386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AAI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105633862023-10-23 Dispensable Role of Aire in CD11c(+) Conventional Dendritic Cells for Antigen Presentation and Shaping the Transcriptome Miyazawa, Ryuichiro Nagao, Jun-ichi Arita-Morioka, Ken-ichi Matsumoto, Minoru Morimoto, Junko Yoshida, Masaki Oya, Takeshi Tsuneyama, Koichi Yoshida, Hideyuki Tanaka, Yoshihiko Matsumoto, Mitsuru Immunohorizons Adaptive Immunity Aire, the defect of which is responsible for the development of autoimmunity, is predominantly expressed in medullary thymic epithelial cells, and it controls a wide variety of genes, including those of tissue-restricted Ags, for establishing thymic tolerance. Aire is also expressed from APCs in the periphery, called extrathymic Aire-expressing cells (eTACs), and their complementing role to thymic tolerance has been suggested. eTACs are composed of two distinct classes of APCs, conventional dendritic cell (cDC)–type and group 3 innate lymphoid cell (ILC3)-like–type expressing retinoic acid receptor–related orphan receptor γt (RORγt). Although the essential role of Aire in the latter in the Th17-mediated immune response against Candida albicans has been reported, the role of Aire in the cDC-type eTACs for this action has not been examined. Furthermore, the significance of Aire in the production of the transcriptome of the cDC-type eTACs remains unknown. We have approached these issues using a high-fidelity Aire-reporter mouse strain. We found that although the cDC-type eTACs dominated ILC3-like–type eTACs in number and they served as efficient APCs for the immune response against an exogenous Ag as well as for the C. albicans–specific Th17 immune response, loss of Aire in cDC-type eTACs showed no clear effect on these functions. Furthermore, loss of Aire showed no major impact on the transcriptome from cDC-type eTACs. These results suggested that Aire in cDC-type eTACs may not have a cell-intrinsic role in the immune response in contrast to the role of Aire in ILC3-like–type eTACs. AAI 2023-01-25 /pmc/articles/PMC10563386/ /pubmed/36695731 http://dx.doi.org/10.4049/immunohorizons.2200103 Text en Copyright © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under the terms of the CC BY-NC-ND 4.0 Unported license (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Adaptive Immunity Miyazawa, Ryuichiro Nagao, Jun-ichi Arita-Morioka, Ken-ichi Matsumoto, Minoru Morimoto, Junko Yoshida, Masaki Oya, Takeshi Tsuneyama, Koichi Yoshida, Hideyuki Tanaka, Yoshihiko Matsumoto, Mitsuru Dispensable Role of Aire in CD11c(+) Conventional Dendritic Cells for Antigen Presentation and Shaping the Transcriptome |
title | Dispensable Role of Aire in CD11c(+) Conventional Dendritic Cells for Antigen Presentation and Shaping the Transcriptome |
title_full | Dispensable Role of Aire in CD11c(+) Conventional Dendritic Cells for Antigen Presentation and Shaping the Transcriptome |
title_fullStr | Dispensable Role of Aire in CD11c(+) Conventional Dendritic Cells for Antigen Presentation and Shaping the Transcriptome |
title_full_unstemmed | Dispensable Role of Aire in CD11c(+) Conventional Dendritic Cells for Antigen Presentation and Shaping the Transcriptome |
title_short | Dispensable Role of Aire in CD11c(+) Conventional Dendritic Cells for Antigen Presentation and Shaping the Transcriptome |
title_sort | dispensable role of aire in cd11c(+) conventional dendritic cells for antigen presentation and shaping the transcriptome |
topic | Adaptive Immunity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563386/ https://www.ncbi.nlm.nih.gov/pubmed/36695731 http://dx.doi.org/10.4049/immunohorizons.2200103 |
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