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CO(2)‐mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation

Enterobacteriaceae are known to proliferate in cucumber juice, deriving energy from the fermentation of sugars to organic acids and ethanol, and theoretically generating carbon dioxide (CO(2)). We hypothesized that the CO(2) produced by the indigenous Enterobacteriaceae in the early stage of cucumbe...

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Autores principales: Zhai, Yawen, Pagán‐Medina, Christian G., Pérez‐Díaz, Ilenys M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563668/
https://www.ncbi.nlm.nih.gov/pubmed/37823135
http://dx.doi.org/10.1002/fsn3.3557
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author Zhai, Yawen
Pagán‐Medina, Christian G.
Pérez‐Díaz, Ilenys M.
author_facet Zhai, Yawen
Pagán‐Medina, Christian G.
Pérez‐Díaz, Ilenys M.
author_sort Zhai, Yawen
collection PubMed
description Enterobacteriaceae are known to proliferate in cucumber juice, deriving energy from the fermentation of sugars to organic acids and ethanol, and theoretically generating carbon dioxide (CO(2)). We hypothesized that the CO(2) produced by the indigenous Enterobacteriaceae in the early stage of cucumber fermentation accumulates in the fermenting fruits causing bloater defect. The ability of seven Enterobacteriaceae, indigenous to cucumber, to grow and produce CO(2) in cucumber juice medium (CJM), a sterile model system for cucumber fermentation, was characterized. The induction of bloater defect in cucumber fermentation conducted with pasteurized and acidified fruits was also evaluated. The generation times of the seven Enterobacteriaceae in CJM ranged between 0.25 and 8.20 h and resulted in carbon dioxide (CO(2)) production to estimated amounts of 7.22–171.5 mM. Enterobacter cancerogenus and Enterobacter nimipressuralis were among the bacteria that produced the most and the least CO(2) in CJM, respectively, at estimated mM concentrations of 171.58 ± 42.96 and 16.85 ± 6.53. Inoculation of E. cancerogenus and E. nimipressuralis in acidified and pasteurized cucumbers resulted in the production of 138 and 27 mM CO(2), respectively. Such Enterobacteriaceae produced 2% hydrogen in the model cucumber fermentations. A bloater index of 25.4 and 17.4 was calculated from the cucumbers fermented by E. cancerogenus and E. nimipressuralis, respectively, whereas no defect was observed in the fruits collected from uninoculated control fermentation jars. It is concluded that the metabolic activity of the Enterobacteriaceae indigenous to cucumber can produce sufficient CO(2) in cucumber fermentations to induce bloater defect.
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spelling pubmed-105636682023-10-11 CO(2)‐mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation Zhai, Yawen Pagán‐Medina, Christian G. Pérez‐Díaz, Ilenys M. Food Sci Nutr Original Articles Enterobacteriaceae are known to proliferate in cucumber juice, deriving energy from the fermentation of sugars to organic acids and ethanol, and theoretically generating carbon dioxide (CO(2)). We hypothesized that the CO(2) produced by the indigenous Enterobacteriaceae in the early stage of cucumber fermentation accumulates in the fermenting fruits causing bloater defect. The ability of seven Enterobacteriaceae, indigenous to cucumber, to grow and produce CO(2) in cucumber juice medium (CJM), a sterile model system for cucumber fermentation, was characterized. The induction of bloater defect in cucumber fermentation conducted with pasteurized and acidified fruits was also evaluated. The generation times of the seven Enterobacteriaceae in CJM ranged between 0.25 and 8.20 h and resulted in carbon dioxide (CO(2)) production to estimated amounts of 7.22–171.5 mM. Enterobacter cancerogenus and Enterobacter nimipressuralis were among the bacteria that produced the most and the least CO(2) in CJM, respectively, at estimated mM concentrations of 171.58 ± 42.96 and 16.85 ± 6.53. Inoculation of E. cancerogenus and E. nimipressuralis in acidified and pasteurized cucumbers resulted in the production of 138 and 27 mM CO(2), respectively. Such Enterobacteriaceae produced 2% hydrogen in the model cucumber fermentations. A bloater index of 25.4 and 17.4 was calculated from the cucumbers fermented by E. cancerogenus and E. nimipressuralis, respectively, whereas no defect was observed in the fruits collected from uninoculated control fermentation jars. It is concluded that the metabolic activity of the Enterobacteriaceae indigenous to cucumber can produce sufficient CO(2) in cucumber fermentations to induce bloater defect. John Wiley and Sons Inc. 2023-07-19 /pmc/articles/PMC10563668/ /pubmed/37823135 http://dx.doi.org/10.1002/fsn3.3557 Text en Published 2023. This article is a U.S. Government work and is in the public domain in the USA. Food Science & Nutrition published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zhai, Yawen
Pagán‐Medina, Christian G.
Pérez‐Díaz, Ilenys M.
CO(2)‐mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation
title CO(2)‐mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation
title_full CO(2)‐mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation
title_fullStr CO(2)‐mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation
title_full_unstemmed CO(2)‐mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation
title_short CO(2)‐mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation
title_sort co(2)‐mediated bloater defect can be induced by the uncontrolled growth of enterobacteriaceae in cucumber fermentation
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563668/
https://www.ncbi.nlm.nih.gov/pubmed/37823135
http://dx.doi.org/10.1002/fsn3.3557
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