Photodynamic reactions using high-intensity red LED promotes gingival wound healing by ROS induction
Photodynamic therapy is a treatment that combines a light source with a photosensitizer. LEDs have attracted considerable attention in clinical dentistry because they are inexpensive and safe to use. Although the interaction between photosensitizers and LEDs in dental practice is effective for treat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10564724/ https://www.ncbi.nlm.nih.gov/pubmed/37816801 http://dx.doi.org/10.1038/s41598-023-43966-2 |
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author | Minagawa, Emika Yamauchi, Nobuhiro Taguchi, Yoichiro Umeda, Makoto |
author_facet | Minagawa, Emika Yamauchi, Nobuhiro Taguchi, Yoichiro Umeda, Makoto |
author_sort | Minagawa, Emika |
collection | PubMed |
description | Photodynamic therapy is a treatment that combines a light source with a photosensitizer. LEDs have attracted considerable attention in clinical dentistry because they are inexpensive and safe to use. Although the interaction between photosensitizers and LEDs in dental practice is effective for treating periodontal disease by killing periodontopathic bacteria, little is known about the effects of LEDs on human gingival fibroblasts (HGnFs), which play an important role in gingival wound healing. In this study, we investigated the effects of high-intensity red LED irradiation on HGnFs after the addition of methylene blue (MB), one of the least harmful photosensitizers, on wound healing and reactive oxygen species (ROS) production induced by photodynamic reactions. We found that irradiation of MB with high-intensity red LED at controlled energy levels promoted cell proliferation, migration, and production of wound healing factors. Furthermore, ROS production by a photodynamic reaction enabled the translocation of phosphorylated Grb2-associated binder-1, activating Extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase signals. Our findings suggest that proper control of ROS production has a beneficial effect on gingival fibroblasts, which constitute periodontal tissue, from the perspective of gingival wound healing. |
format | Online Article Text |
id | pubmed-10564724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105647242023-10-12 Photodynamic reactions using high-intensity red LED promotes gingival wound healing by ROS induction Minagawa, Emika Yamauchi, Nobuhiro Taguchi, Yoichiro Umeda, Makoto Sci Rep Article Photodynamic therapy is a treatment that combines a light source with a photosensitizer. LEDs have attracted considerable attention in clinical dentistry because they are inexpensive and safe to use. Although the interaction between photosensitizers and LEDs in dental practice is effective for treating periodontal disease by killing periodontopathic bacteria, little is known about the effects of LEDs on human gingival fibroblasts (HGnFs), which play an important role in gingival wound healing. In this study, we investigated the effects of high-intensity red LED irradiation on HGnFs after the addition of methylene blue (MB), one of the least harmful photosensitizers, on wound healing and reactive oxygen species (ROS) production induced by photodynamic reactions. We found that irradiation of MB with high-intensity red LED at controlled energy levels promoted cell proliferation, migration, and production of wound healing factors. Furthermore, ROS production by a photodynamic reaction enabled the translocation of phosphorylated Grb2-associated binder-1, activating Extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase signals. Our findings suggest that proper control of ROS production has a beneficial effect on gingival fibroblasts, which constitute periodontal tissue, from the perspective of gingival wound healing. Nature Publishing Group UK 2023-10-10 /pmc/articles/PMC10564724/ /pubmed/37816801 http://dx.doi.org/10.1038/s41598-023-43966-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Minagawa, Emika Yamauchi, Nobuhiro Taguchi, Yoichiro Umeda, Makoto Photodynamic reactions using high-intensity red LED promotes gingival wound healing by ROS induction |
title | Photodynamic reactions using high-intensity red LED promotes gingival wound healing by ROS induction |
title_full | Photodynamic reactions using high-intensity red LED promotes gingival wound healing by ROS induction |
title_fullStr | Photodynamic reactions using high-intensity red LED promotes gingival wound healing by ROS induction |
title_full_unstemmed | Photodynamic reactions using high-intensity red LED promotes gingival wound healing by ROS induction |
title_short | Photodynamic reactions using high-intensity red LED promotes gingival wound healing by ROS induction |
title_sort | photodynamic reactions using high-intensity red led promotes gingival wound healing by ros induction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10564724/ https://www.ncbi.nlm.nih.gov/pubmed/37816801 http://dx.doi.org/10.1038/s41598-023-43966-2 |
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