Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR

Organoids, combined with genetic editing strategies, have the potential to offer rapid and efficient investigation of gene function in many models of human disease. However, to date, the editing efficiency of organoids with the use of non-viral electroporation methods has only been up to 30%, with i...

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Detalles Bibliográficos
Autores principales: Skoufou-Papoutsaki, Nefeli, Adler, Sam, D'Santos, Paula, Mannion, Liz, Mehmed, Shenay, Kemp, Richard, Smith, Amy, Perrone, Francesca, Nayak, Komal, Russell, Alasdair, Zilbauer, Matthias, Winton, Douglas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Resource Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565108/
https://www.ncbi.nlm.nih.gov/pubmed/37772705
http://dx.doi.org/10.1242/dmm.050279

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565108/
https://www.ncbi.nlm.nih.gov/pubmed/37772705
http://dx.doi.org/10.1242/dmm.050279

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