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Increasing the bulk of the 1TEL–target linker and retaining the 10×His tag in a 1TEL–CMG2-vWa construct improves crystal order and diffraction limits

TELSAM-fusion crystallization has the potential to become a revolutionary tool for the facile crystallization of proteins. TELSAM fusion can increase the crystallization rate and enable crystallization at low protein concentrations, in some cases with minimal crystal contacts [Nawarathnage et al. (2...

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Detalles Bibliográficos
Autores principales: Gajjar, Parag L., Pedroza Romo, Maria J., Litchfield, Celeste M., Callahan, Miles, Redd, Nathan, Nawarathnage, Supeshala, Soleimani, Sara, Averett, Jacob, Wilson, Elijah, Lewis, Andrew, Stewart, Cameron, Tseng, Yi-Jie, Doukov, Tzanko, Lebedev, Andrey, Moody, James D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565734/
https://www.ncbi.nlm.nih.gov/pubmed/37747038
http://dx.doi.org/10.1107/S2059798323007246