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InterOpt: Improved gene expression quantification in qPCR experiments using weighted aggregation of reference genes

qPCR is still the gold standard for gene expression quantification. However, its accuracy is highly dependent on the normalization procedure. The conventional method involves using the geometric mean of multiple study-specific reference genes (RGs) expression for cross-sample normalization. While re...

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Detalles Bibliográficos
Autores principales: Salimi, Adel, Rahmani, Saeid, Sharifi-Zarchi, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565776/
https://www.ncbi.nlm.nih.gov/pubmed/37829204
http://dx.doi.org/10.1016/j.isci.2023.107945