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InterOpt: Improved gene expression quantification in qPCR experiments using weighted aggregation of reference genes
qPCR is still the gold standard for gene expression quantification. However, its accuracy is highly dependent on the normalization procedure. The conventional method involves using the geometric mean of multiple study-specific reference genes (RGs) expression for cross-sample normalization. While re...
Autores principales: | Salimi, Adel, Rahmani, Saeid, Sharifi-Zarchi, Ali |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565776/ https://www.ncbi.nlm.nih.gov/pubmed/37829204 http://dx.doi.org/10.1016/j.isci.2023.107945 |
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