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MILKSHAKE: novel validation method for antibodies to post-translationally modified targets by surrogate Western blot

Antibody (Ab) validation is the procedure in which an Ab is thoroughly assayed for sensitivity and specificity in a given application. Validation of Abs against post-translationally modified (PTM) targets is particularly challenging because it requires specifically prepared antigen. Here we describe...

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Detalles Bibliográficos
Autores principales: Jones, Kezzia S, Chapman, Amanda E, Driscoll, Holland A, Fuller, Emily P, Kelly, Meghan, Li, Xiaofeng, Mansour, Sourour, McBride, Shannon L, Zhao, Qi, Weiner, Michael, Ferguson, Mary R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568613/
https://www.ncbi.nlm.nih.gov/pubmed/34841898
http://dx.doi.org/10.2144/btn-2021-0078