A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiation

The C2C12 cell line represents a simple in vitro model for cell differentiation. Here, we present a flow-cytometry-based pipeline to quantitate C2C12 cell differentiation based on myosin heavy-chain marker expression. We describe steps for cell seeding, transfection, drug treatment, differentiation,...

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Detalles Bibliográficos
Autores principales: Parisi, Bianca, Sünnen, Maxime, Chippalkatti, Rohan, Abankwa, Daniel Kwaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568640/
https://www.ncbi.nlm.nih.gov/pubmed/37819762
http://dx.doi.org/10.1016/j.xpro.2023.102637

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568640/
https://www.ncbi.nlm.nih.gov/pubmed/37819762
http://dx.doi.org/10.1016/j.xpro.2023.102637

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