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A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiation
The C2C12 cell line represents a simple in vitro model for cell differentiation. Here, we present a flow-cytometry-based pipeline to quantitate C2C12 cell differentiation based on myosin heavy-chain marker expression. We describe steps for cell seeding, transfection, drug treatment, differentiation,...
Autores principales: | Parisi, Bianca, Sünnen, Maxime, Chippalkatti, Rohan, Abankwa, Daniel Kwaku |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568640/ https://www.ncbi.nlm.nih.gov/pubmed/37819762 http://dx.doi.org/10.1016/j.xpro.2023.102637 |
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