Unidirectional trans-cleaving behavior of CRISPR-Cas12a unlocks for an ultrasensitive assay using hybrid DNA reporters containing a 3′ toehold

CRISPR-Cas12a can induce nonspecific trans-cleavage of dsDNA substrate, including long and stable λ DNA. However, the mechanism behind this is still largely undetermined. In this study, we observed that while trans-activated Cas12a didn’t cleave blunt-end dsDNA within a short reaction time, it could...

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Detalles Bibliográficos
Autores principales: Mohammad, Noor, Talton, Logan, Hetzler, Zach, Gongireddy, Megha, Wei, Qingshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570054/
https://www.ncbi.nlm.nih.gov/pubmed/37650631
http://dx.doi.org/10.1093/nar/gkad715

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570054/
https://www.ncbi.nlm.nih.gov/pubmed/37650631
http://dx.doi.org/10.1093/nar/gkad715

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